Graft-versus-host disease (GVHD) is one of the greatest risks patients undergoing allogenic hematopoietic stem cell transplantation face; it results in serious and sometimes fatal complications in the approximately 30 percent of patients who reject the transplant. Traditionally, doctors have relied on methods to match major human leukocyte antigens (HLA) in patients and donors to determine compatibility, which work in most cases. However, differences in minor antigens?the number and identity of which is unclear?often generate histocompatibility complications that result in GVHD.
We thinks the most practical way to discover the minor antigens involved in GVHD may be to use high-density genotyping microarrays. Ogawa is performing a 2000-patient case-control association study with an early version of the Affymetrix 500K array to genotype over 500,000 SNPs from each participant.
This project is
supported by Core Research for Evolutional Science and
Technology (CREST), Japan Science and Technology Agenecy.
Cancer death accounts for one third of annual mortality in Japan and other developed countries. Cancer develops based on alterations of the genome. We are exploring genetic changes in cancer genomes using high-density oligonucleotide microarrays developed by Affymetrix in combination with greatly improved algorithm for detecting copy number/LOH/allelic imbalances. See also CNAG software package.
Leuekemia/Lymphoma
Gastric cancer
Colorectal cancer
Hepatoma
Pancreatic cancer
Lung cancer
Breast cancer
Brain tumors
Ovarian cancer
Cancer in childhood
A. Copy number analysis
CNAG
CNAG View
CNAG View2
B. New SNP call program on Affymetrix GeneChip platform
C. Power estimation of WGA studies